In short: No. You can update your publication with new data at any time. So you don't have to delete your publication first and then upload a new publication. In fact, this is not recommended. Genealogy Online keeps various data for each publication, including an explanatory text, starting points, stories, suggestions for online scans and suggestions for starting points in other publications. If you delete your publication, all these things will also be deleted!
To update your publication in terms of data, do the following:
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