(1) Hij is getrouwd met Barbara de Meij.
Toestemming voor het huwelijk is 11 juli 1654 verkregen te Delft.Bron 1
Zij zijn getrouwd op 29 juli 1654 te Delft, hij was toen 18 jaar oud.Bron 1(2) Hij is getrouwd met Cornelia Swalmius.
Toestemming voor het huwelijk is 10 januari 1671 verkregen te Delft.Bron 2
Zij zijn getrouwd op 25 januari 1671 te Pijnacker, hij was toen 35 jaar oud.Bron 3Anno 1671
january
Den 25e dito in den echten staat bevesticht Anthony Leeuwenhoeck weduwnaer en Cornelia Swalmius j.d. beyde tot Delft
"To Antonio van Leeuwenhoek, of Delft, belongs the high merit of having been the first to use the microscope systematically and of having brought the construction of the simple microscope in his own hands to a high degree of perfection . . . Self-taught and never having attended a university, ignorant of Latin and Greek and of the classical texts, he became one of the greatest and most expert microscopists, thanks to the sagacity of his observations and the perfection of his technique"
(Arturo Castiglioni, A history of medicine).
On July 29, 1654, he married Barbara de Mey, daughter of Elias de May, a serge merchant from Norwich, England. Of their five children only one, their daughter Maria, survived her father. Barbara died in 1666.
Leeuwenhoek remarried on January 25, 1671. His second wife was Cornelia Swalmius, the daughter of Johannes Swalmius, a Calvinist minister at Valkenburg, near Leiden. She died in 1694.