 Attention: Died 9 months (May  1, 1539) prior to the birth (February 24, 1545) of child (John of HABSBURG) .
 Attention: Died 9 months (May  1, 1539) prior to the birth (February 24, 1545) of child (John of HABSBURG) .
 Attention: Wife (Charles V of HABSBURG) is also his cousin.
 Attention: Wife (Charles V of HABSBURG) is also his cousin.
She had a relationship with Charles V of HABSBURG.
Child(ren):
 grandparents
 grandparents  
 parents
 parents  
 brothers/sisters
 brothers/sisters  
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 children
| Isabella of PORTUGAL | |||||||||||||||||||||||||||||||||||||||
| Charles V of HABSBURG | |||||||||||||||||||||||||||||||||||||||