Irene is a city in Clay, Turner, and Yankton counties in the U.S. state of South Dakota. The population was 437 at the 2010 census. The portion of Irene that is located in Turner County is a part of the Sioux Falls, South Dakota metropolitan area, while the portion located in Yankton County is part of the Yankton Micropolitan Statistical Area. Irene is also host to the Irene Rodeo, held every summer.